H7N2禽流感病毒分离株血凝素蛋白全基因的序列分析

为进一步分析H7N2禽流感病毒（AIV）分离株血凝素（HA）基因的特性,参照已发表序列设计了1对引物,采用RT-PCR获得了1条约1.7 kb的DNA片段,测序后进行了同源性比较、HA基因系统发育进化树分析和氨基酸编码分析.结果表明,所测的2个分离株的HA基因全长1 664 bp,编码除信号肽以外的HA蛋白的全部544个氨基酸,其中包括HA1的323个氨基酸,HA2的221个氨基酸.2个分离株HA基因核苷酸序列的同源性为99.4%;与GenBank中AIV标准株A/Afri.Star./Eng-Q/983/79（H7N1）的同源性最高,分别为99.4%和99.0%;与美国A/Chicken/NewYork/13142-5/94（H7N2）株同源性很低（仅65.0%）,而与以色列、意大利H7N2 AIV的同源性较高（为96%～97%）;2个分离株在HA基因进化树中均处于H7亚型AIV的欧亚群系分支内.推导氨基酸的序列分析表明,其HA蛋白裂解位点的氨基酸序列为-GR-GLF-,仅包含1个碱性氨基酸（R-）残基,符合低致病力AIV的基因特征.     

新疆山地鸟类和哺乳类多样性及其区系特征

新疆各类陆栖脊椎动物近 70 0种 ,占中国这一类动物种数的 32 %,其中鸟兽资源尤其丰富 ,计有 42 5种鸟类和 1 5 5种兽类 (哺乳类 ) ,占中国鸟兽种数的 31 %。本文主要以新疆三大山区———阿尔泰山、天山、昆仑 -阿尔金山地区鸟类和哺乳类动物的多样性及区系分布作综合性介绍和分析。根据实地考察和以往文献资料 ,新疆阿尔泰山区记录在 1 2 4种鸟类 ,其中古北界北方型鸟类 5 1种 (占 41 .1 %) ,中亚型鸟类 31种 (占 2 5 .0 %) ,广布种鸟类 2 7种 (占 2 1 .8%) ,其他类型1 5种 (占 1 2 .1 %) ,显出以北方型泰加林鸟类为主的鸟类区系特征 ;分布的 5 0种兽类 ,区系上以古北界北方泰加林为主 ,即北方型兽类占该山区种数的 5 2 %。天山山区记录的鸟类大约2 81种 ,北方型种类占绝对优势 ,即北方型 1 0 6种 (占 37.72 %) ,北方广布型 76种 (占 2 7.0 4 %) ,中亚型 39种 (占 1 3.88%) ,高地型 1 8种 (占 6.41 %) ,广布型 33种 (占 1 1 .74%) ,东北型 9种 (占 3.2 1 %)。兽类分布有 62种 ,北方型兽类区系占优势 ,有 30种 (占 48.40 %) ,广布型 1 2种 (占 1 9.40 %) ,中亚型 1 1种 (占 1 7.70 %) ,特有种 9种 (占 1 4.5 0 %)。昆仑—阿尔金山地区 96种鸟类中 ,中亚类型成份占 2 8.1 %,高地型成分占 2 6%,北方型成?     

Effect of moxonidine hydrochloride on left ventricular hypertrophy in spontaneously hypertensive rats

AIM:To investate the effect of domestric moxonidine hydrochloride on myocardium fibrosis and coronary artery microvascular structure in left ventricular hypertrophy of spontaneously hypertensive rats(SHR).METHODS:30 male SHR, aged 20 weeks, were divided into group Mox+SHR, Cap+SHR and SHR randomly (10 in each group). 10 age and sex-mached sprauge-dawley rats were designed as normal control(NC). At the end of 13 weeks, left ventricular wight/body weight ratio(LVW/BW), collagen volume fraction(CVF) and standardized perivascular collagen area(PVCA) as well as intramyocardial arterial average medial thickness (AMT) were determined.RESULTS:LVW/BW, CVF, PVCA and AMT in group Mox+SHR were lower significantly than that in group SHR, respectively.CONCLUSION:Long-term antihypertensive treatment with moxonidine hydrochloride reduces myocardium fibrosis and improves impaired coronary artery microvascular structure in left ventricular hypertrophy.     

‘三棱榄''橄榄果实香气成分分析

1 材料与方法选取广东优良鲜食橄榄品种‘三棱榄’,2001年12月6日采样,采用固相微萃取法(SPME)富集香气成分(鲜橄榄果肉于15℃下捣碎后取样1.0 g放入4 mL聚四氟乙烯硅橡胶垫密封螺口玻璃瓶中,插入100μm聚二甲基硅氧烷纤维头于室温25-30℃顶空取样2 h),用美国Finnigan TRACE GC-MS气相色谱-质谱联用仪进行分析。气相色谱柱为DB-1弹性毛细管柱30 m×0.25 mm,载气为He(99.99％),流速1.0mL/min。程序升温从40℃开始先保持10 min,后以2℃/min的升温速率升至150℃保持10 min。质谱条件:电子能量70 eV,离子源温度250℃,质量范围35-450 aum,不分流进样。2002年12月18日采样重复分析。     

Effects of Sini decoction on atherosclerosis and ceramide content of aorta in rabbits

AIM:To observe the effects of Sini decoction on atherosclerosis(AS) and ceramide content of aorta in rabbits. METHODS:28 rabbits were randomly divided into three groups. Control group was fed with a normal diet; High cholesterol group was fed 1% cholesterol and 5% fat diet; Sini decotion+ high cholesterol group was fed 1% cholesterol and 5% fat diet plus Sini decotion (4.2 g·kg^-1·d^-1). At the end of study, the plaque area were measured, the atorta ceramide and cell apoptosis were also detected. RESULTS:Sini decotion diminished lipid plaque area on the aortic endothelium, reduced the levels of aorta ceramide and the apoptosis index. CONCLUSION:Sini decoction has an inhibitory effect on AS, the mechanism may be that Sini decotion reduces concentration of ceramide in aorta.     

新疆棉区转Bt基因棉对棉田主要害虫及其天敌种群数量的影响

20 0 0～ 2 0 0 1年 ,在新疆棉区系统地研究了转Bt基因棉田主要害虫及其天敌的发生规律。结果表明 :转Bt基因棉对棉铃虫有良好的控制作用 ,发生高峰期百株落卵量和百株幼虫数量都显著低于常规对照 ,转Bt自控田和转Bt化防田棉蚜和棉盲蝽发生数量则显著高于常规棉对照田。在天敌方面 ,转Bt自控田和转Bt化防田较常规棉对照田 ,丽草蛉种群数量有所增加 ,而小花蝽和黑食蚜盲蝽等 5种捕食性和几种寄生性天敌数量都有所下降。转Bt基因棉田棉蚜和棉盲蝽等刺吸性害虫发生数量加重而多种天敌数量减少的趋势值得引起重视。鉴于新疆地区棉铃虫发生数量较低目前无需专门防治的特点 ,作者建议 :可以考虑推广农艺性状较好的常规棉品种并保护天敌 ,以达到控制害虫、丰产丰收的目的。     

灰树花不同菌株液体深层培养蛋白质含量的测定

在液体培养条件下测定了发酵液中5个灰树花菌株蛋白质含量的变化。结果表明，接种后第6天发酵液中蛋白质含量最高，Gf961菌株发酵液中的蛋白质含量为0.58%，Gf962菌株发酵液中的蛋白质含量最低，为0.30%，随着培养时间的延长，发酵液中蛋白质含量逐渐下降，接种后第9天最低，随后以呈上升趋势。但上升的幅度较小。试验为确定从发酵液中提取蛋白质的最佳时期提供了一定的理论依据。     

Expression of alpha-smooth muscle actin in scar tissue and its relationship with apoptosis

AIM: To examine the expression of alpha-smooth muscle actin in scar tissue, and observe the phenomenon of apoptosis and its involvement in the process of pathological scarring and the presence of myofibroblasts or absence of cell in the dermis. To investigate the potential role of reparative cell apoptosis in hyperplastic scar formation. METHODS: The samples of scar were obtained from post-burn patients undergoing plastic operation in our burn unit recently, and the samples of control came from skin donor site of the same patient correspondingly. TUNEL assays were performed to evaluate the number of apoptotic cells in scar versus normal skin. In situ hybridization and immunohistochemistry staining technique were employed to determine the expression of different dermis cells markers in scar tissue and normal skin. RESULTS: There existed evident difference in apoptotic cells in the dermis between scars tissue and normal human skin. The expression positive cells were much more in hyperplastic scars than that in normal human skin; the apoptotic cells of proliferative stage were slight more than that of mature stage. However, in proliferative stage, the number of apoptotic cells was higher for the combination of hyperplastic scar than normally healed flat scars. But in mature stage, no obviously difference was detected between hyperplastic scar and normally healed flat scar. The monoclonal anti-α smooth muscle actin (ASMA) expression was significantly stronger in proliferative stage than that of mature stage. CONCLUSIONS: With reconstitution of dermal tissue, myofibroblasts containing alpha-SM actin disappear under normal wound healing, probably as a result of apoptosis. The myofibroblast play a critical role in wound closure and in the pathologic sequelae of healing.     

Influences of chronic hypoxia on the gene expression of Kv1.3,Kv2.1 and Kv3.1 induced by acute hypoxia in PASMC of rats

AIM and METHODS: Total RNA was extracted from 6th rat subcultured pulmonary artery smooth muscle cells(PASMC) exposed to continual chronic hypoxia or normoxia and the effects of chronic hypoxia on the changes of Kv1.3,Kv2.1,Kv3.1 mRNA in cultured PASMC induced by acute hypoxia were studied by semiquantitative RT-PCR in vitro. RESULTS:①Kv1.3,Kv2.1,Kv3.1 genes were found to be expressed in PASMC of rats exposed either to hypoxia or normxia.②The expression of Kv2.1 and Kv3.1 in 6th subcultured of PASMC in normaxia group could be upregulated by exposure to acute hypoxia,the levels of Kv2.1 and Kv3.1 mRNA were significantly increased from 0.646±0.092, 0.782±0.104 to 1.059±0.134, 0.985±0.116,respectively (P<0.01,n=5). ③PASMC cultured continuously in chronic hypoxia for 6 subcultures and then exposed to normoxia for 12 h,thereafter the expression of Kv2.1 and Kv3.1 were downregulated by acute hypoxia for 6 hours.The level of Kv2.1 mRNA was significantly decreased from 1.008±0.117 to 0.649±0.097 (P<0.01,n=5). CONCLUSION:Kv2.1,Kv3.1 genes might be oxygen sensitive genes.Chronic hypoxia might change the response of these Kv genes of PASMC to acute hypoxia and down-regulate its expression,which might probably decrease the role of Kv in HPV.     

Relationship between expression of somatostatin and DNA ploidy in breast cancer

AIM: To investigate the relationship between somatostatin and the pathologic type, estrogen receptor,DNA ploidy of nuclei in tumor cells of breast cancer.METHODS: 67 cases of primary breast cancer and 25 cases of benign breast tumor were examined by immunohistochemical stretomyces avidin peroxidase method. 26 cases of breast cancer selected at random were analyzed by flow cytometry.RESULTS: Somatostatin expressed significantly higher in low malignant breast cancer than that in high malignant breast cancer (P<0.05). Most of cancers with positive staining of somatostatin were diploidy,most of cancers with negative staining were aneuploidy,there had significant difference between two groups (P<0.05).CONCLUSION: Somatostatin may delay the progress of breast cancer,and somatostatin levels in cancer tissues may become a useful indicator for assessing prognosis of patients with breast cancer.